Microorganisms represent the major portion of biomass on Earth with enormous diversity in morphology, genetics, and metabolism, which implies huge application potentials for humankind. Our group is interested in using genetic, genomic, and bioinformatic approaches to develop microbial systems that can be applied to various environmental processes, such as production of biofuels and green chemicals, remediation of hazardous substances, and environmental detection and monitoring. Currently, we focus on a group of hyperthermophilic bacteria called Thermotoga. These bacteria can ferment sugars to acetic acid and release hydrogen gas as a waste, which may be harvested by humans to be used as a clean fuel or an intermediate for many industrial applications. Their robust thermostable enzymes have also drawn wide attention from the biotechnology sector. Please visit our lab page to learn more about us.
(Student authors†; corresponding authors*)
Puranik P†, Quan G, Werner J†, Zhou R, Xu Z*. A pipeline for completing bacterial genomes using in silicoand wet lab approaches. BMC Genomics. 2014.
Xu H†, Han D†, Xu Z*. Overexpression of a lethal methylase M.TneDI in E. coli BL21(DE3).Biotechnology Letters. 36(9): 1853-1859. 2014.
Han D†, Xu H†, Puranik R†, Xu Z*. Natural transformation of Thermotoga sp. strain RQ7. BMC Biotechnology. 14:39. 2014.
Han D†, SM Norris†, Xu Z*. Construction and transformation of a Thermotoga-E. coli shuttle vector. BMC Biotechnology. 12:2. 2012.
Xu Z*, Han D†, Cao J†, Saini, U. Cloning and characterization of the TneDI restriction-modification system of Thermotoga neapolitana. Extremophiles. 15(6):665-72. 2011.
Xu Z*, Dutton RJ, Gober JW. Direct interaction of FliX and FlbD is required for their regulatory activity in Caulobacter crescentus. BMC Microbiology. 11:89. 2011.
Ditty J, Kvaal C, Goodner B, Freyermuth S, Bailey C, Britton R, Gordon S, Heinhorst S, Reed K, Xu Z, Sanders-Lorenz E, Axen S, Kim E, Johns M, Scott K, Kerfeld, C*. Faculty incorporating genomics and bioinformatics across the life sciences curriculum: development and implementation of the integrated microbial genomes annotation collaboration toolkit (IMG-ACT). PLoS Biology. 8(8): e1000448. 2010.
Xu Z*, Lei Y, Patel J†. Bioremediation of soluble heavy metals with recombinant Caulobacter crescentus.Bioengineered Bugs (now: Bioengineered). 1 (3):207-12. 2010.
Patel J†, Wilson G†, McKay RML, Vincent R, Xu Z*. Self-immobilization of recombinant Caulobacter crescentus and its application in removal of cadmium from water. Applied Biochemistry and Biotechnology. 162(4):1160-1173. 2010.
Patel J†, Zhang Q, McKay RML, Vincent R, Xu Z*. Genetic engineering of Caulobacter crescentus for removal of cadmium from water. Applied Biochemistry and Biotechnology. 160 (1): 232-243. 2010.