H. Peter Lu

H. Peter Lu, Ph.D.

Ohio Eminent Scholar and Professor, Chemistry Dept.
021 Overman Hall
419.372.1840
hplu@bgsu.edu
Research Group Page


Biographical Facts:

Joined the faculty in 2006
Ph.D., Columbia University (1991)
M.S., Peking University, P.R. China (1984)

Research Interests:

Our research is focused on the use of single molecule techniques to understand molecular dynamic processes and the effects of the local environment on these processes. We have been developing and applying time-resolved, nanoscale site-specific, single molecule methods that are an effective alternative to conventional methods, providing information under conditions most applicable to the natural processes underlying the area of research interest. Single-molecule approaches are useful and unique in studying heterogeneous and complex systems because the inhomogeneity can be identified and/or removed by studying one molecule at a time. Single molecules and molecular complexes can be observed as they traverse a wide range of energy states in real-time and the effect of this ever changing "system configuration" on chemical/biological reactions and other dynamical processes can be mapped.

Our current research work has been focused on (1) conformational dynamics and reaction in proteins and protein complexes under physiological conditions, and our long-term goal is to study single-molecule protein conformational dynamics and reactions in living cells; and (2) inhomogeneous interfacial chemical and biological reaction dynamics in solar energy conversion, bioremediation, and environmental systems, focusing on fundamental understanding of the controlling physical and chemical properties, such as, Franck-Condon coupling and barrier, vibrational and solvent relaxation energetics, molecular distributions, redox states identification, and molecular motions.

Selected Publications:

Zijiang Wang, H. Peter Lu, "Single-Molecule Spectroscopy Study of Crowding-Induced Protein Spontaneous Denature and Crowding-Perturbed Unfolding-Folding Conformational Fluctuation Dynamics," J. Phys. Chem. B,  122, 6724-6732 (2018).

S. Roy Chowdhury, H. Peter Lu, "Probing Activated and Non-Activated Single Calmodulin Molecule under a picoNewton Compressive Force," Biochemistry,  57, 1945-1948 (2018).

S. Roy Chowdhury, Jin Cao, Yufan He, and H. Peter Lu, "Revealing Abrupt and Spontaneous Ruptures of Protein Native Structure under Pico-Newton Compressive Stress Force Manipulation," ACS Nano,  12, 2448-2454 (2018).

Achut P. Silwal, Rajeev Yadav, Jon E. Sprague, and H. Peter Lu, "Raman Spectroscopic Signature Markers of Dopamine-Human Dopamine Transporter Interaction in Living Cells," ACS Chemical Neuroscience,  8, 1510-1518 (2017).

Vishal G. Rao, H. Peter Lu, "Inhomogeneous and Complex Interfacial Electron-Transfer Dynamics: A Single-Molecule Perspective," ACS Energy Lett., invited review article,  1, 773-791 (2016).

Nibedita Pal, Meiling Wu, H. Peter Lu, "Probing Conformational Dynamics of Enzymatic Active Site by an In Situ Single Fluorogenic Probe under picoNewton Force Manipulation," Proc. Natl. Acad. Sci.113, 15006-15011 (2016).

Rajeev Yadav, and H. Peter Lu, "Probing the Dynamic Heterogeneity in the Aggregated Ion Channels in Live Cells," J. Phys. Chem. C,  122, 13716-13723 (2018).

Rajeev Yadav, and H. Peter Lu, "Revealing Dynamically-Organized Receptor Ion Channel Clusters in Live Cells by a Correlated Electric Recording and Super-Resolution Single-Molecule Imaging," Phys. Chem. Chem. Phys.,  20, 8088-8098 (2018).

Meiling Wu, Rajeev Yadav, Nibedita Pal, and H. Peter Lu, "Manipulating Motions of Targeted Single Cells in Solution by an Integrated Double-Ring Magnetic Tweezers Imaging Microscope," Rev. Sci. Instrum.,  88, 07373 (2017).

Bharat Dhital, Vishal Govind Rao, and H. Peter Lu, "Probing Single-Molecule Electron-Hole Transfer Dynamics at a Molecule-NiO Semiconductor Nanocrystalline Interface," Phys. Chem. Chem. Phys.,  19, 17216-17223 (2017).

Achut P. Silwal, Rajeev Yadav, Jon E. Sprague, and H. Peter Lu, "Raman Spectroscopic Signature Markers of Dopamine-Human Dopamine Transporter Interaction in Living Cells," ACS Chemical Neuroscience,  8, 1510-1518 (2017).

Nibedita Pal, Meiling Wu, H. Peter Lu, "Probing Conformational Dynamics of Enzymatic Active Site by an In Situ Single Fluorogenic Probe under picoNewton Force Manipulation," Proc. Natl. Acad. Sci.113, 15006-15011 (2016).

Dibyendu Sasmal, Rajeev Yadav, H. Peter Lu, "Single-Molecule Patch-Clamp-FRET-Anisotropy Microscopy Studies of NMDA Receptor Ion Channel Activation and Deactivation under Agonist Ligand Binding in Living Cells," J. Am. Chem. Soc.,  138, 8789-8801 (2016).

Yufan He, Vishal G. Rao, Jin Cao, H. Peter Lu, "Simultaneous Spectroscopic and Topographic Imaging of Single-Molecule Interfacial Electron Transfer Reactivity and Local Nanoscale Environment," J. Phys. Chem. Lett.,  7, 2221-2227 (2016).

Bharat Dhital, Vishal G. Rao, H. Peter Lu, "Electronic Coupling-Decoupling Dependent Single-Molecule Interfacial Electron Transfer Dynamics in Electrostatically-Attached Porphyrin on TiO2 Nanoparticles," J. Phys. Chem. C,  120, 12313-12324 (2016).

Vishal G. Rao, Bharat Dhital, H. Peter Lu, "Probing Driving Force and Electron Accepting State Density Dependent Interfacial Electron Transfer Dynamics: Suppressed Fluorescence Blinking of Single-Molecules on Indium Tin Oxide Semiconductors," J. Phys. Chem. B.,  120,1685-1697 (2016).