Fluorescence
Web page: dated September 18, 2002
This is the normal color of three samples
- 1= the dye fluorescein (yellow, absorbs blue light)
- 2= the dye called rhodamine (pink, absorbs green light)
- 3= quinine (tonic water) (colorless, absorbs no visible light)
When exposed to UV light all three species glow
When exposed to the blue light only two samples glow
- remember that quinine is colorless
- absorbs no visible light (including blue)
- therefore it won't acquire energy and can't fluorescence
The path of light is easily seen in a larger cell of fluorescein
- light is, of course, only absorbed in the beam of the LED source
- therefore light is only emitted fromthe same portion of the solution